Cloning, expression and antifungal effect of the recombinant chitinase from Streptomyces sampsonii KJ40

ABSTRACT: Streptomyces sampsonii is a kind of biocontrol bacterium with antifungal effects, and chitinase one the main substances. To improve further study structure function gene S. sampsonii, we amplified target fragment by PCR, ligated to expression vector pET-32a, introduced resulting plasmid into Escherichia coli BL21 (DE3) induced chitinase. Then, recombinant was purified is-labelled protein micro purification kit. A gene, Sschi61, cloned from genome expressed in prokaryote. The effect also studied. Finally, Sschi61 length 1755 bp obtained, 82 kDa E. IPTG. could inhibit black spot pathogen Eucommia ulmoides (Pestalotiopsis trachicarpicola). After hyphae trachicarpicola) were soaked chitinase, cells expanded, broke, dissolved.